Anopheles gambiae Control Potential and Antibacterial Activity of Opuntia ficus-indica (Cactus Plant) Mediated Nanoparticles
Article Sidebar
Main Article Content
Abstract
Infected mosquitoes transmit malaria globally, and their resistance to insecticides has sparked a global search for biological control strategies. This study investigated the insecticidal and antibacterial potential of previously greensynthesised and characterised silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), and silver-gold alloy nanoparticles (Ag-AuNPs) derived from Opuntia ficus-indica (OF). Larvicidal and pupicidal effects were tested at 1-30 µg/ml concentrations over 12 to 24h. Simultaneously, the impact of nanoparticle-infused coil fumes on adult mosquitoes was assessed at 170 µg/ml. Antibacterial activity was evaluated using the agar well diffusion method against eight clinical isolates at concentrations ranging from 10–50 µg/ml. The results showed that mortality rates in larvae and pupae increased with concentration over time, with the highest effect observed at 170 µg/ml in adult mosquitoes exposed to fumes. Antibacterial evaluations showed that the nanoparticles exhibited zones of inhibition between 5 and 12 mm against eight clinical bacterial isolates, with OF-AuNPs and OF-Ag-AuNPs being particularly effective against Pseudomonas aeruginosa. Overall, the findings highlight the potential of OF-derived nanoparticles for larvicidal, pupicidal, adulticidal, and antibacterial applications in insect and bacterial control, respectively.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Sato S.(2021). Plasmodium-a brief introduction to the parasites causing human malaria and their basic biology. Journal of Physiological Anthropology, 40(1), 1. https://doi.org/10.1186/s40101-020-00251-9
Abdi, A. I., Müller, R., Obi, I., Nyonda, M., Oyieko, J., Amwayi, P., Ouattara, A., Barry, A., Coulibaly, M., Niaré, O., Traoré, K., Traoré, O., Coulibaly, B., Dicko, A., Sauerwein, R., Bousema, T., Dechering, K. J., Lanke, K., & Stone, W. (2023). Plasmodium falciparum adapts it's investment into replication versus transmission according to the host environment. eLife, 12, e85140. DOI: 10.7554/eLife.85140
World Health Organisation (2023). World Malaria Report 2023. World Health Organisation. https://cdn.who.int/media/docs/default-source/malaria/world-malaria-reports/world-malaria-report-2023.pdf
Adebayo, E. A., Oke, M. A., Aina, D. A., Fadimu, A. O., & Olatunde, O. O. (2021). Antioxidant potential of the biosynthesized silver, gold and silver-gold alloy nanoparticles using Opuntia ficus-indica extract. Fountain Journal of Natural and Applied Sciences, 10(2), 28–38. DOI: 10.53704/fujnas.v10i2.354
Nova, I. C. V., de Almeida, W. A., Procópio, T. F., Ferreira, J. D. L., do Nascimento, T. G., do Nascimento, D. D., Oliveira, M. S., & Maciel, M. C. G. (2022). Extract from Opuntia ficus-indica cladode delays the Aedes aegyptilarval development by inducing an Axenic midgut environment. Archives of Insect Biochemistry and Physiology, 113(1), e21872. https://doi.org/10.1002/arch.21872
Amrane-Abider, M., Imre, M., Herman, V., Debbou-Iouknane, N., Saci, F., Boudries, H., Madani, K., Merzouk, H., & Ayad, A. (2023). Opuntia ficus-indica peel by product as a natural antioxidant food additive and anticoccidial Drug. Foods, 12(24), 4403. DOI:10.3390/foods12244403
Wang, J., Rani, N., Jakhar, S., Redhu, R., Kumar, S., Kumar, R., Devi, B., Simal-Gandara, J., Shen, B., & Singla, R. K. (2023). Opuntia ficus-indica (L.) Mill. Anticancer properties and phytochemicals: current trends and future perspectives. Frontiers in Plant Science, 14, Article 1236123. DOI: 10.3389/fpls.2023.1236123
Lu, A. H., Salabas, E. L., & Schüth, F. (2008). Magnetic nanoparticles: Synthesis, protection, functionalization, and application. Angewandte Chemie International Edition, 46(8), 1222-1248. DOI: 10.1002/anie.200602866
Husen, A., & Siddiqi, K. S. (2014). Phytosynthesis of nanoparticles: Concept, controversies and application. Nanoscale Research Letters, 9(1), 1-24. DOI: 10.1186/1556-276X-9-229
Benelli, G., Pavela, R., Canale, A., Mehlhorn, H., & Maggi, F. (2017). Mosquito control with green nanopesticides: Towards sustainable vector management. Environmental Science and Pollution Research, 24(20), 17353-17367. DOI: 10.1007/s11356-017-9752-4
Rizwan, M. (2020). Green synthesis and antimicrobial evaluation of silver nanoparticles: effects of dark incubation. Microsystems & Nanoengineering, 6, 23. DOI:10.1049/mnl.2019.0617
Martins, M., Ribeiro, M. H., & Almeida, C. M. M. (2023). Physicochemical, nutritional, and medicinal properties of Opuntia ficus-indica (L.) Mill and its main agro-industrial use: A review. Plants, 12(7), 1512. DOI: 10.3390/plants12071512
Adebayo, E. A., Abel, M. O., Oke, A., Lateef, A., Abayomi, A., Oyatokun, O. D., Abisoye, I. P., Adiji, D. O., Fagbenro, T. V., Amusan, B. J. A., Asafa, T. B., Beukes, L. S., Gueguim-Kana, E. B., & Abbas, S. H. (2019a). Biosynthesis of silver, gold, and silver-gold alloy nanoparticles using Persea americana fruit peel aqueous extract for their biomedical properties. Nanotechnology for Environmental Engineering. DOI: 10.1007/s41204-019-0060-8.
Balogun, H. A., & Ajala, O. O. (2018). Effect of phytochemical components of the leaf of Moringa oleifera on the development of Anopheles gambiae. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(6): 64 71. Doi:10.26538/tjpps/v3i6.2
Zavala-Zapata, V., Ramírez-Barrón, S.N., Sánchez-Borja, M., Aguirre-Uribe, L.A., Delgado-Ortiz,J.C., Sánchez-Peña, S.R., Mayo-Hernández, J., García-López, J.I., Vargas-Tovar, J.A., & Hernández-Juárez, A. (2024). Insecticide efficacy of green synthesis silver nanoparticles on Diaphorina citri Kuwayama (Hemiptera: Liviidae). Insects, 15(7): 469. Doi: 10.3390/insects15070469
Adebayo, E. A., Ibikunle, J. B., Abel, M. O., Lateef, A., Musbau, A. A., Adeboye, O. O., Ajala, V. A., Olowoporoku, T. B., Okunlola, O. C., Ogundele, O. A., Badmus, A. B., Asafa, T. B., Beukes, L. S., Gueguim-Kana, E. B., & Abbas, S. H. (2019). Antimicrobial and antioxidant activity of silver, gold, and silver-gold alloy nanoparticles photosynthesised using extract of Opuntia ficus-indica. Review on Advanced Materials Science, 58:313–326.DOI:10.1515/rams-2019-0039.
Selim, Y. A., Azb, M. A., Ragab, I., & Abd El-Azim, M. H. M. (2020). Green Synthesis of Zinc Oxide Nanoparticles Using Aqueous Extract of Deverra tortuosa and their Cytotoxic Activities. Scientific Reports, 10, 18638. DOI: 10.1038/s41598-020-60541-1.
Kumari, M., Mishra, A., & Pandey, S. (2015). Green synthesis and characterisation of silver nanoparticles using leaf extract of Azadirachta indica.Applied Nanoscience, 5(8), 1175-1182. DOI:10.1007/s13204-015-0453-9
Eid, A. M.(2022). Biosynthesis of noble metal nanoparticles using plant extracts and their applications. Nanomaterials, 12(2), 241. DOI:10.3390/nano12020241
Kuppan, P., Sundaravadivelu, M., & Munusamy, S. (2015). Green synthesis of silver nanoparticles using plantextract and their antimicrobial activity: Areview. Journal of Nanomaterials, 2015, 1-8. DOI:10.1155/2015/756873
Roopan, S. M., Bharathi, A., & Raj, M. (2013). Eco-friendly synthesis of silver nanoparticles using Aloe vera leaf extract and evaluation of their larvicidal potential against Anopheles stephensi. Journal of Molecular Liquids, 181, 77-82. DOI:10.1016/j.molliq.2013.03.014
Priyadarshini, R., Selvan, G., & Venkatesan, B. (2012). Biogenic synthesis of silver nanoparticles using Psidium guajava leaf extract and their activity against mosquito larvae. Asian Pacific Journal of Tropical Disease, 2, S399-S403.DOI:10.1016/S2222- 1808(12)60100-2
Suresh, P., Vasanthakumar, V., & Mohan, J. (2018). Green synthesis of silver nanoparticles using Azadirachta indica leaves and their applications in controlling Aedes aegypti larvae. Journal of Cluster Science, 29(4), 653-660. DOI: 10.1007/s10876-017-1313-y
Benelli, G., & Mehlhorn, H. (2017). Declining malaria, rising of dengue and Zika virus: Insights for mosquito vector control. Parasitology Research, 116, 1617-1621. DOI:10.1007/s00436-017-5450-1
Awad, M. A., Hassan, S. M., & El-Mokhtar, M. A.(2022). Green synthesis of silver nanoparticles using Opuntia ficus-indica for larvicidal and pupicidal activities. International Journal of Environmental Research and Public Health, 19(6), 1-12. DOI:10.3390/ijerph19063778
Hassan, D. H., Ahmed, M. I., & Youssef, M. A. (2021). Larvicidal activity of silver nanoparticles synthesised from Opuntia ficus-indica against Anopheles gambiae. Egyptian Journal of Biological Pest Control, 31(3), 110. DOI:10.1186/s41938-021-00379-9
Manimegalai, S., Chitra, S., & Mohamed, M. H. (2020). Antimosquito activity of green-synthesised silver nanoparticles against Aedes aegypti larvae and pupae. Environmental Nanotechnology, Monitoring and Management, 14, 100336. DOI: 10.1016/j.enmm.2020.100336
Aziz, A. (2023). Efficacy of nanoparticle-based insecticides: A review. Journal of Applied Entomology, 147(3), 253-261.DOI: 10.1111/jen.13007
Haldar, S., Chattopadhyay, D., & Mukherjee, S. (2014). Toxicological effects of silver nanoparticles on Anopheles stephensi. Journal of Toxicology and Environmental Health,77(13),786-795. DOI:10.1080/15287394.2014.888415
Sondi, I., & Salopek-Sondi, B.(2004). Silver nanoparticles as antimicrobial agents: A case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 275(1), 177-182. DOI: 10.1016/j.jcis.2004.02.012
Gogoi, S. K., Gopinath, P., & Paul, A. (2006). Green synthesis of silver nanoparticles using bacterial isolates: An eco-friendly approach. Journal of Bacteriology and Nanotechnology, 12(4), 329-336. DOI: 10.1016/j.nantod.2006.07.001
Jain, P., Pradeep, T., & Pandey, R. (2015). Mechanisms of antibacterial activity of biogenic silver nanoparticles synthesised using plant extracts. Journal of Nanoscience and Nanotechnology, 15(1), 1-12. DOI: 10.1166/jnn.2015.10221