Serological Evidence of Dengue Virus Exposure and Possible Active Infection in Stored Serum Samples from a University Medical Centre in Ilara-Mokin, Southwest Nigeria
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Abstract
Dengue virus infection is a major global public health concern; however, its true burden in many parts of sub-Saharan Africa, including Nigeria, remains under-recognised and often misdiagnosed, particularly in malaria-endemic settings. This pilot surveillance study aimed to investigate the serological evidence of dengue virus exposure and active infection in a university community in southwest Nigeria. A total of 106 stored serum samples from patients who sought care at Elizade University Medical Centre were analysed. Samples were tested for dengue virus NS1 antigen (NS1Ag) and IgG/IgM antibodies using the SD BIOLINE Dengue Duo rapid diagnostic kit. Descriptive statistics were used to analyse the demographic data and prevalence of dengue markers. Overall, 22.64% (24/106) of individuals whose stored serum samples were analysed showed serological evidence of dengue virus infection (any marker positive). One individual (0.94%) tested positive for NS1Ag, indicating an acute infection. IgM antibodies, suggestive of recent infection, were detected in nine individuals (8.49%), while IgG antibodies, indicating past exposure, were found in 22 individuals (20.75%). Males showed a positivity rate (27.94%; 19/68) compared to females (13.16%; 5/38). Among the 90 participants with age data, younger age groups (<20 years: 30.00%; 20-29 years: 26.00%) showed higher positivity. Among the 92 participants with known states of origin, Ondo State residents showed the highest positivity (33.33%; 9/27), followed by Bayelsa (29.17%; 7/24) and Delta (17.14%; 6/35). This pilot study provides serological evidence of both recent and past dengue virus infections in the Ilara-Mokin university community, suggesting local transmission and a notable level of exposure to the virus. These findings underscore the need for increased awareness, enhanced surveillance, and the integration of dengue diagnostics into routine febrile illness management in Nigeria to accurately determine its prevalence and mitigate its public health impact. Further studies, including molecular characterisation, are required.
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References
Bhatt, S., Gething, P. W., Brady, O. J., Messina, J. P., Farlow, A. W., Moyes, C. L., Drake, J. M., Brownstein, J. S., Hoen, A. G., Sankoh, O., Myers, M. F., George, D. B., Jaenisch, T., Wint, G. R. W., Simmons, C. P., Scott, T. W., Farrar, J. J., & Hay, S. I. (2013). The global distribution and burden of dengue. Nature, 496(7446), 504–507. https://doi.org/10.1038/nature12060
World Health Organization. (2024). Dengue – Global situation. https://www.who.int/emergencies/disease-outbreak-news/item/2024-DON518
World Health Organization. (2021). Dengue and severe dengue. https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue
Yadav, A., Shamim, U., Ravi, V., Devi, P., Kumari, P., Maurya, R., Das, P., Somani, M., Budhiraja, S., Tarai, B., & Pandey, R. (2023). Early transcriptomic host response signatures in dengue patients. Scientific Reports, 13(1), 14170. https://doi.org/10.1038/s41598-023-41205-2
Simmons, C. P., Farrar, J. J., Nguyen, V. V. C., & Wills, B. (2012). Dengue. New England Journal of Medicine, 366(15), 1423–1432. https://doi.org/10.1056/NEJMra1110265
Hales, S., de Wet, N., Maindonald, J., & Woodward, A. (2002). Potential effect of population and climate changes on global distribution of dengue fever. The Lancet, 360(9336), 830–834. https://doi.org/10.1016/S0140-6736(02)09964-6
Ayukekbong, J. A., Oyero, O. G., Nnukwu, S. E., Mesumbe, H. N., & Fobisong, C. N. (2017). Value of routine dengue diagnosis in endemic countries. World Journal of Virology, 6(1), 9–16. https://doi.org/10.5501/wjv.v6.i1.9
Okoror, L., Bankefa, E. O., Ukhureigbe, O. M., Ajayi, E. O., Osanyilusi, S., & Ogeneh, B. (2024). Misdiagnosis of dengue fever as malaria and typhoid fever in rural Nigeria. Qeios, 6(5). https://doi.org/10.32388/cj7d8i.2
Linda, O. E., Chimere, E. V., & George, O. C. (2021). Dengue virus IgM seroprevalence and malaria parasitaemia in HIV-infected individuals. Journal of Advances in Microbiology, 21, 17–29. https://doi.org/10.9734/jamb/2021/v21i1230410
Animasaun, O. S., Shaibu, J. O., Akomolafe, B. K., et al. (2025). Enhancing surveillance for dengue fever in Oyo State, Nigeria: A One Health approach. One Health Outlook, 7(1), 5. https://doi.org/10.1186/s42522-024-00121-9
Adesola, R. O., Ajibade, F. A., Idris, I., Scott, G. Y., & Agaie, M. I. (2024). Addressing dengue fever challenges in Nigeria: A narrative review. Le Infezioni in Medicina, 32(2), 157–167. https://doi.org/10.53854/liim-3202-5
Bhatt, P., Sabeena, S. P., Varma, M., & Arunkumar, G. (2021). Pathogenesis of dengue virus infection. Current Microbiology, 78(1), 17–32. https://doi.org/10.1007/s00284-020-02284-w
Otu, A., Ebenso, B., Etokidem, A., & Chukwuekezie, O. (2019). Dengue fever update and implications for Nigeria. African Health Sciences, 19(2), 2000–2007. https://doi.org/10.4314/ahs.v19i2.23
Faneye, A., Idika, N., Motayo, O. B., Adesanmi, A., & Afocha, E. (2013). Dengue virus infection among febrile children. American Journal of Infectious Diseases, 9(1), 7–10. https://doi.org/10.3844/ajidsp.2013.7.10
Baba, M., Logue, C. H., Oderinde, B., et al. (2013). Arbovirus co-infection in febrile malaria/typhoid patients. Journal of Infection in Developing Countries, 7(1), 51–59. https://doi.org/10.3855/jidc.2411
Baba, M. M., Saron, M.-F., Vorndam, A. V., et al. (2009). Dengue virus infections in Nigeria. Journal of American Science, 5(5), 129–134. https://doi.org/10.7537/marsjas050509.16
Onyedibe, K., Dawurung, J., Iroezindu, M., et al. (2018). Dengue virus infection in febrile patients in Nigeria. Malawi Medical Journal, 30(4), 276–282. https://doi.org/10.4314/mmj.v30i4.11
Anker, M., & Arima, Y. (2011). Gender differences in dengue cases in Asia. Western Pacific Surveillance and Response Journal, 2(2), 17–23. https://doi.org/10.5365/WPSAR.2011.2.1.002
Guzman, M. G., Halstead, S. B., et al. (2010). Dengue: A global threat. Nature Reviews Microbiology, 8(12 Suppl), S7–S16. https://doi.org/10.1038/nrmicro2460
Nema, D. S., Deol, D. A., Tripathi, D. K., & Ramnani, D. V. K. (2017). Seroepidemiology of dengue in Bhopal. IOSR Journal of Dental and Medical Sciences, 16(1), 95–99. https://doi.org/10.9790/0853-1601049599