Effects of Melatonin, Vitamins C and E Supplement on Antibody Response to Newcastle Disease Vaccinated Ascaridia galli Infected Broiler Chickens 1
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Abstract
This study was conducted to determine the effects of melatonin, vitamin C, and vitamin E supplementation on the antibody response to Newcastle disease vaccinated Ascaridia galli-infected broiler chickens. A total of one-hundred and eighty, day-old broilers were randomly allotted into six groups (A–F) of 30 birds each: A (A. galli, no vaccination), B (A. galli + NDV), C (A. galli + NDV + vitamin C 250 mg daily), D (A. galli + NDV + vitamin E 250 mg daily), E (A. galli + NDV + melatonin 0.5 mg daily), and F (NDV only; uninfected control). A dose of 1000 infective A. galli eggs was administered orally, while ND vaccine was administered at day 1i/o), day 21 (La Sota, p.o.), and day 42 (Komarov, i/m). The birds were weighed weekly, and blood samples were collected to determine the antibody response to Newcastle disease vaccination. After infection, the McMaster technique was used to determine the faecal egg count. The mean weight (kg) increase of groups E, D, and C (2.32 ± 0.00, 2.18 ± 0.00, 2.07 ± 0.00) were significantly higher (P<0.001) than that of groups A (1.64 ± 0.00Kg) and B (1.74 ± 0.01Kg), but significantly lower than that of group F (2.66 ± 0.0Kg). At the termination of the experiment on day 54, groups C, D, and E had significantly lower faecal egg count (380 ± 0.0, 240 ±1.6, 300 ± 1.4) than groups A (1300 ± 2.1) and B (1200 ± 0.0). From days 28 to 56, groups C, D, and E birds had significantly higher antibody titres than groups B and F birds. The respective values at day 56 were 2.27 ± 0.20, 2.36 ± 0.18, 2.50 ± 0.2, 1.32 ± 0.29 and 1.43 ± 0.22. Between days 35 and 56, Group B had significantly lower antibody titres than Group F. Ascaridia galli infection negatively affected the antibody response to Newcastle disease vaccination.
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